Bioengineering a non-genotoxic vector for genetic modification of mesenchymal stem cells.

ABSTRACT: Vectors used for stem cell transfection must be non-genotoxic, in addition to possessing high efficiency, because they could potentially transform normal stem cells into cancer-initiating cells. The objective of this research was to bioengineer an efficient vector that can be used for genetic modification of stem cells without any negative somatic or genetic impact. Two types of multifunctional vectors, namely targeted and non-targeted were genetically engineered and purified from E. coli. The targeted vectors were designed to enter stem cells via overexpressed receptors. The non-targeted vectors were equipped with MPG and Pep1 cell penetrating peptides. A series of commercial synthetic non-viral vectors and an adenoviral vector were used as controls. All vectors were evaluated for their e

SUBMITTER: Chen X 

PROVIDER: S-EPMC5671363 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature