Forkhead box protein 3 demethylation is associated with tolerance induction in peanut-induced intestinal allergy.

ABSTRACT:

Background

Regulatory T (Treg) cells play an essential role in the maintenance of immune homeostasis in allergic diseases.

Objectives

We sought to define the mechanisms underlying induction of tolerance to peanut protein and prevention of the development of peanut allergy.

Methods

High or low doses of peanut extract were administered to pups every day for 2 weeks before peanut sensitization and challenge. After challenge, symptoms, Treg cell numbers, and forkhead box protein 3 (Foxp3), T_H2 and T_H17 cytokine, and Tgfβ expression in mesenteric lymph node (MLN) CD4⁺ T cells and jejunum were monitored. Treg cell suppressive activity and Foxp3 methylation in MLN CD4⁺ T cells were assayed.

Results

Feeding high but not low d

SUBMITTER: Wang M 

PROVIDER: S-EPMC5671381 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature