Development of an efficient and reproducible regeneration system in wheat (Triticum aestivum L.).

ABSTRACT: The availability of reproducible regeneration system through tissue culture is a major bottleneck in wheat improvement program. The present study has considered to develop an efficient callus induction and regeneration system using mature and immature embryos as explants in recently released agronomically superior spring wheat varieties. An efficient sterilization process was standardized using 0.1% HgCl2 and 70% ethanol for both seeds and embryos. The maximum possible combinations of plant growth regulators (PGRs) were evaluated for their effect on different wheat regeneration processes through tissue culture starting from callus to root induction. Picloram is found as an effective auxin with 87.63-98.67% callus induction efficiency in both explants. Supplementation of CuSO4 along with 2,

SUBMITTER: Kumar R 

PROVIDER: S-EPMC5671445 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature