Generation of an Attenuated Tiantan Vaccinia Virus Strain by Deletion of Multiple Genes.

ABSTRACT: An attenuated vaccinia virus-MVTT_EAB-was constructed by deletion of non-essential gene segments related to the immunomodulatory and virulence functions of the vaccinia virus Tiantan strain (VVTT). The shuttle plasmids pTC-EGFP, pTE-EGFP, pTA35-EGFP, pTB-EGFP, and pTA66-EGFP were constructed and combined with the early and late strong promoter pE/L and EGFP as an exogenous selectable marker. Then, through the homologous recombination technology and Cre/loxP system, the following gene fragments were gradually knocked out one by one: TC7L-TK2L, TE3L, TA35R, TB13R, and TA66R. Ultimately, the five-segment-deleted attenuated strain MVTT_EAB was obtained. Knockout of these segments and genetic stability of MVTT_EAB were confirmed, and it was also shown that knockout

SUBMITTER: Li Y 

PROVIDER: S-EPMC5671601 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature