Development of an Indirect ELISA and Dot-Blot Assay for Serological Detection of Rice Tungro Disease.

ABSTRACT: Rice tungro disease (RTD) is one of the most destructive diseases of rice in South and Southeast Asia. RTD is routinely detected based on visual observation of the plant. However, it is not always easy to identify the disease in the field as it is often confused with other diseases or physiological disorders. Here we report the development of two serological based assays for ease of detection of RTD. In this study we had developed and optimized an indirect ELISA and dot-blot assay for detection of RTD. The efficiency of both assays was evaluated by comparing the specificity and sensitivity of the assays to PCR assay using established primer sets. The indirect ELISA showed 97.5% and 96.6%, while the dot-blot assay showed 97.5% and 86.4% sensitivity and specificity, respectively, when compar

SUBMITTER: Henry Sum MS 

PROVIDER: S-EPMC5671674 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature