Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.

ABSTRACT: Membrane-associated proteins are required for essential processes like transport, organelle biogenesis, and signaling. Many are expected to function as part of an oligomeric protein complex. However, membrane-associated proteins are challenging to work with, and large-scale data sets on the oligomerization state of this important class of proteins is missing. Here we combined cell fractionation of Arabidopsis leaves with nondenaturing detergent solubilization and LC/MS-based profiling of size exclusion chromatography fractions to measure the apparent masses of >1350 membrane-associated proteins. Our method identified proteins from all of the major organelles, with more than 50% of them predicted to be part of a stable complex. The plasma membrane was the most highly enriched in large prote

SUBMITTER: McBride Z 

PROVIDER: S-EPMC5672003 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature