¹H-Detected REDOR with Fast Magic-Angle Spinning of a Deuterated Protein.

ABSTRACT: Rotational echo double resonance (REDOR) is a highly successful method for heteronuclear distance determination in biological solid-state NMR, and ¹H detection methods have emerged in recent years as a powerful approach to improving sensitivity and resolution for small sample quantities by utilizing fast magic-angle spinning (>30 kHz) and deuteration strategies. In theory, involving ¹H as one of the spins for measuring REDOR effects can greatly increase the distance measurement range, but few experiments of this type have been reported. Here we introduce a pulse sequence that combines frequency-selective REDOR (FSR) with ¹H detection. We demonstrate this method with applications to samples of uniformly ¹³C,¹⁵N,²H-labeled ala

SUBMITTER: Ghosh M 

PROVIDER: S-EPMC5673488 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature