1,25-Dihydroxy vitamin D₃ stimulates system A amino acid transport in primary human trophoblast cells.

ABSTRACT: Vitamin D deficiency during pregnancy is linked to adverse perinatal outcomes such as small for gestational age infants. Recent evidence suggests that changes in placental amino acid transport contribute to altered fetal growth. We tested the hypothesis that 1,25-dihydroxy vitamin D₃ increases the gene expression of System A and L amino acid transporter isoforms and stimulates placental amino acid transport activity in cultured primary human trophoblast cells mediated by mTOR signaling. Treatment with 1,25-dihydroxy vitamin D₃ significantly increased mRNA expression of the System A isoform SNAT2 and System A activity, but had no effect on System L and did not affect mTOR signaling. siRNA silencing of the vitamin D receptor prevented 1,25-dihydroxy vitamin D₃

SUBMITTER: Chen YY 

PROVIDER: S-EPMC5673492 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature