Red blood cell minor antigen mismatches during chronic transfusion therapy for sickle cell anemia.

ABSTRACT:

Background

Red blood cell (RBC) alloimmunization occurs at a high frequency in sickle cell anemia (SCA) despite serologic matching for Rh (C/c, E/e) and K antigens. RBC minor antigen genotyping allows for prediction of antigens and RH variants that may lead to alloimmunization.

Study design and methods

RBC antigen genotyping was performed on chronically transfused pediatric SCA patients, using PreciseType human erythrocyte antigen (HEA), RHCE, and RHD BeadChip arrays. All patients received C/c, E/e, and K serologically matched units (Category 1); patients with prior RBC antibodies were also matched for Fy^a , Jk^b , and any antibodies (Category 2). The RBC genotypes of all leukoreduced (LR) units transfused over a 12-month period were determined by the pr

SUBMITTER: Yee MEM 

PROVIDER: S-EPMC5673534 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature