Single-Molecule Fluorescence Methods to Study Plant Hormone Signal Transduction Pathways.

ABSTRACT: Plant-hormone-initiated signaling pathways are extremely vital for plant growth, differentiation, development, and adaptation to environmental stresses. Hormonal perception by receptors induces downstream signal transduction mechanisms that lead to plant responses. However, conventional techniques-such as genetics, biochemistry, and physiology methods-that are applied to elucidate these signaling pathways can only provide qualitative or ensemble-averaged quantitative results, and the intrinsic molecular mechanisms remain unclear. The present study developed novel methodologies based on in vitro single-molecule fluorescence assays to elucidate the complete and detailed mechanisms of plant hormone signal transduction pathways. The proposed methods are based on multicolor total internal refle

SUBMITTER: Song S 

PROVIDER: S-EPMC5673658 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature