Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2.

ABSTRACT:

Background

Canine parvovirus 2, a linear single-stranded DNA virus belonging to the genus Parvovirus within the family Parvoviridae, is a highly contagious pathogen of domestic dogs and several wild canidae species. Early detection of canine parvovirus (CPV-2) is crucial to initiating appropriate outbreak control strategies. Recombinase polymerase amplification (RPA), a novel isothermal gene amplification technique, has been developed for the molecular detection of diverse pathogens. In this study, a real-time RPA assay was developed for the detection of CPV-2 using primers and an exo probe targeting the CPV-2 nucleocapsid protein gene.

Results

The real-time RPA assay was performed successfully at 38 °C, and the results were obtained within 4-12 min for 10⁵-10

SUBMITTER: Geng Y 

PROVIDER: S-EPMC5674863 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature