Shared αβ TCR Usage in Lungs of Sarcoidosis Patients with Lofgren's Syndrome.

ABSTRACT: Sarcoidosis is a granulomatous disease that primarily affects the lungs and is characterized by an accumulation of CD4⁺ T cells in the bronchoalveolar lavage (BAL). Previous work has indicated that HLA-DRB1*03:01⁺ (DR3⁺) patients diagnosed with the acute form of the disease, Löfgren's syndrome (LS), have an accumulation of CD4⁺ T cells bearing TCRs using TRAV12-1 (formerly AV2S3). However, the importance of these α-chains in disease pathogenesis and the paired TCRβ-chain remains unknown. This study aimed to identify expanded αβTCR pairs expressed on CD4⁺ T cells derived from the BAL of DR3⁺ LS patients. Using a deep-sequencing approach, we determined TCRα- and TCRβ-chain usage, as well as αβTCR pairs expressed on BAL CD4

SUBMITTER: Mitchell AM 

PROVIDER: S-EPMC5675165 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature