Three-dimensional automated reporter quantification (3D-ARQ) technology enables quantitative screening in retinal organoids.

ABSTRACT: The advent of stem cell-derived retinal organoids has brought forth unprecedented opportunities for developmental and physiological studies, while presenting new therapeutic promise for retinal degenerative diseases. From a translational perspective, organoid systems provide exciting new prospects for drug discovery, offering the possibility to perform compound screening in a three-dimensional (3D) human tissue context that resembles the native histoarchitecture and to some extent recapitulates cellular interactions. However, inherent variability issues and a general lack of robust quantitative technologies for analyzing organoids on a large scale pose severe limitations for their use in translational applications. To address this need, we have developed a screening platform that enables a

SUBMITTER: Vergara MN 

PROVIDER: S-EPMC5675442 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature