An analysis of the gene interaction networks identifying the role of PARP1 in metastasis of non-small cell lung cancer.

ABSTRACT:

Background and objective

Though there were many researches about the effects of cancer cells on non-small cell lung cancer (NSCLC) currently, it has been rarely reported completed oncogene and its mechanism in tumors by far. Here, we used biological methods with known oncogene of NSCLC to find new oncogene and explore its functionary mechanism in NSCLC.

Methods

The study firstly built NSCLC genetic interaction network based on bioinformatics methods and then combined shortest path algorithm with significance test to confirmed core genes that were closely involved with given genes; real-time qPCR was conducted to detect expression levels between patients with NSCLC and normal people; additionally, detection of PARP1's role in migration and invasion was performed by trans-well

SUBMITTER: Chen K 

PROVIDER: S-EPMC5675631 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature