Discovery of stimulation-responsive immune enhancers with CRISPR activation.

ABSTRACT: The majority of genetic variants associated with common human diseases map to enhancers, non-coding elements that shape cell-type-specific transcriptional programs and responses to extracellular cues. Systematic mapping of functional enhancers and their biological contexts is required to understand the mechanisms by which variation in non-coding genetic sequences contributes to disease. Functional enhancers can be mapped by genomic sequence disruption, but this approach is limited to the subset of enhancers that are necessary in the particular cellular context being studied. We hypothesized that recruitment of a strong transcriptional activator to an enhancer would be sufficient to drive target gene expression, even if that enhancer was not currently active in the assayed cells. Here we de

SUBMITTER: Simeonov DR 

PROVIDER: S-EPMC5675716 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature