Sequence-specific DNA solid-phase extraction in an on-chip monolith: Towards detection of antibiotic resistance genes.

ABSTRACT: Antibiotic resistance of bacteria is a growing problem and presents a challenge for prompt treatment in patients with sepsis. Currently used methods rely on culturing or amplification; however, these steps are either time consuming or suffer from interference issues. A microfluidic device was made from black polypropylene, with a monolithic column modified with a capture oligonucleotide for sequence selective solid-phase extraction of a complementary target from a lysate sample. Porous properties of the monolith allow flow and hybridization of a target complementary to the probe immobilized on the column surface. Good flow-through properties enable extraction of a 100μL sample and elution of target DNA in 12min total time. Using a fluorescently labeled target oligonucleotide related to Ver

SUBMITTER: Knob R 

PROVIDER: S-EPMC5675797 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature