Spexin Suppress Food Intake in Zebrafish: Evidence from Gene Knockout Study.

ABSTRACT: Spexin1 (SPX1) is a newly discovered neuropeptide in vertebrates. Its biological function remains to be elucidated. In this study, we have generated the zebrafish spx1 ^-/- mutant lines using transcription activator-like effector nucleases. Phenotypes of the spx1 ^-/- mutant zebrafish were analyzed in order to understand the effects on reproduction and food intake. The reproductive capability is not impaired in spx1 mutant zebrafish. However, we found that the spx1 ^-/- mutant fish had a higher food intake than the wild type (WT) fish. Real-time PCR revealed that the expression level of agouti-relate protein 1 (AgRP1), a significant appetite stimulant, was significantly higher in spx1 ^-/- mutant fish after feeding. Intracranial administration of SPX

SUBMITTER: Zheng B 

PROVIDER: S-EPMC5677112 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature