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First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans).

ABSTRACT: Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.

SUBMITTER: Luo R 

PROVIDER: S-EPMC5677167 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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First Draft Genome Sequence of the Pathogenic Fungus <i>Lomentospora prolificans</i> (Formerly <i>Scedosporium prolificans</i>).

Luo Ruibang R   Zimin Aleksey A   Workman Rachael R   Fan Yunfan Y   Pertea Geo G   Grossman Nina N   Wear Maggie P MP   Jia Bei B   Miller Heather H   Casadevall Arturo A   Timp Winston W   Zhang Sean X SX   Salzberg Steven L SL  

G3 (Bethesda, Md.) 20171106 11

Here we describe the sequencing and assembly of the pathogenic fungus <i>Lomentospora prolificans</i> using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chrom  ...[more]

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