Using Linkage Maps as a Tool To Determine Patterns of Chromosome Synteny in the Genus Salvelinus.

ABSTRACT: Next generation sequencing techniques have revolutionized the collection of genome and transcriptome data from nonmodel organisms. This manuscript details the application of restriction site-associated DNA sequencing (RADseq) to generate a marker-dense genetic map for Brook Trout (Salvelinus fontinalis). The consensus map was constructed from three full-sib families totaling 176 F₁ individuals. The map consisted of 42 linkage groups with a total female map size of 2502.5 cM, and a total male map size of 1863.8 cM. Synteny was confirmed with Atlantic Salmon for 38 linkage groups, with Rainbow Trout for 37 linkage groups, Arctic Char for 36 linkage groups, and with a previously published Brook Trout linkage map for 39 linkage groups. Comparative mapping confirmed the presen

SUBMITTER: Hale MC 

PROVIDER: S-EPMC5677171 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature