Expression and targeting of transcription factor ATF5 in dog gliomas.

ABSTRACT:

Background

Activating transcription factor 5 (ATF5) is a transcription factor that is highly expressed in undifferentiated neural progenitor/stem cells as well as a variety of human cancers including gliomas.

Aims

In this study, we examined the expression and localization of ATF5 protein in canine gliomas, and targeting of ATF5 function in canine glioma cell lines.

Materials and methods

Paraffin-embedded canine brain glioma tissue sections and western blots of tumours and glioma cells were immunoassayed with anti-ATF5 antibody. Viability of glioma cells was tested with a synthetic cell-penetrating ATF5 peptide (CP-d/n ATF5) ATF5 antagonist.

Results

ATF5 protein expression was in the nucleus and cytoplasm and was present in normal adult brain and tumour samples,

SUBMITTER: York D 

PROVIDER: S-EPMC5677578 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature