Human single chain-transbodies that bound to domain-I of non-structural protein 5A (NS5A) of hepatitis C virus.

ABSTRACT: A safe and broadly effective direct acting anti-hepatitis C virus (HCV) agent that can withstand the viral mutation is needed. In this study, human single chain antibody variable fragments (HuscFvs) to conserved non-structural protein-5A (NS5A) of HCV were produced by phage display technology. Recombinant NS5A was used as bait for fishing-out the protein bound-phages from the HuscFv-phage display library. NS5A-bound HuscFvs produced by five phage transfected-E. coli clones were linked molecularly to nonaarginine (R9) for making them cell penetrable (become transbodies). The human monoclonal transbodies inhibited HCV replication in the HCVcc infected human hepatic cells and also rescued the cellular antiviral immune response from the viral suppression. Computerized simulation verified by im

SUBMITTER: Glab-Ampai K 

PROVIDER: S-EPMC5678119 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature