PTEN Regulates PI(3,4)P₂ Signaling Downstream of Class I PI3K.

ABSTRACT: The PI3K signaling pathway regulates cell growth and movement and is heavily mutated in cancer. Class I PI3Ks synthesize the lipid messenger PI(3,4,5)P₃. PI(3,4,5)P₃ can be dephosphorylated by 3- or 5-phosphatases, the latter producing PI(3,4)P₂. The PTEN tumor suppressor is thought to function primarily as a PI(3,4,5)P₃ 3-phosphatase, limiting activation of this pathway. Here we show that PTEN also functions as a PI(3,4)P₂ 3-phosphatase, both in vitro and in vivo. PTEN is a major PI(3,4)P₂ phosphatase in Mcf10a cytosol, and loss of PTEN and INPP4B, a known PI(3,4)P₂ 4-phosphatase, leads to synergistic accumulation of PI(3,4)P₂, which correlated with increased invadopodia in epidermal growth factor (EGF

SUBMITTER: Malek M 

PROVIDER: S-EPMC5678281 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature