Redox Changes During the Cell Cycle in the Embryonic Root Meristem of Arabidopsis thaliana.

ABSTRACT:

Aims

The aim of this study was to characterize redox changes in the nuclei and cytosol occurring during the mitotic cell cycle in the embryonic roots of germinating Arabidopsis seedlings, and to determine how redox cycling was modified in mutants with a decreased capacity for ascorbate synthesis.

Results

Using an in vivo reduction-oxidation (redox) reporter (roGFP2), we show that transient oxidation of the cytosol and the nuclei occurred at G1 in the synchronized dividing cells of the Arabidopsis root apical meristem, with reduction at G2 and mitosis. This redox cycle was absent from low ascorbate mutants in which nuclei were significantly more oxidized than controls. The cell cycle-dependent increase in nuclear size was impaired in the ascorbate-deficient mutants, which had

SUBMITTER: de Simone A 

PROVIDER: S-EPMC5678362 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature