Metabolic engineering of Ustilago trichophora TZ1 for improved malic acid production.

ABSTRACT: Ustilago trichophora RK089 has been found recently as a good natural malic acid producer from glycerol. This strain has previously undergone adaptive laboratory evolution for enhanced substrate uptake rate resulting in the strain U. trichophora TZ1. Medium optimization and investigation of process parameters enabled titers and rates that are able to compete with those of organisms overexpressing major parts of the underlying metabolic pathways. Metabolic engineering can likely further increase the efficiency of malate production by this organism, provided that basic genetic tools and methods can be established for this rarely used and relatively obscure species. Here we investigate and adapt existing molecular tools from U. maydis for use in U. trichophora. Sele

SUBMITTER: Zambanini T 

PROVIDER: S-EPMC5678829 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature