Direct interaction with 14-3-3γ promotes surface expression of Best1 channel in astrocyte.

ABSTRACT:

Background

Bestrophin-1 (Best1) is a calcium-activated anion channel (CAAC) that is expressed broadly in mammalian tissues including the brain. We have previously reported that Best1 is expressed in hippocampal astrocytes at the distal peri-synaptic regions, called microdomains, right next to synaptic junctions, and that it disappears from the microdomains in Alzheimer's disease mouse model. Although Best1 appears to be dynamically regulated, the mechanism of its regulation and modulation is poorly understood. It has been reported that a regulatory protein, 14-3-3 affects the surface expression of numerous membrane proteins in mammalian cells.

Methods

The protein-protein interaction between Best1 and 14-3-3γ was confirmed by yeast-two hybrid assay and BiFC method. The effect

SUBMITTER: Oh SJ 

PROVIDER: S-EPMC5679146 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature