Hypoxia-enhanced adhesion of red blood cells in microscale flow.

ABSTRACT:

Objectives

The advancement of microfluidic technology has facilitated the simulation of physiological conditions of the microcirculation, such as oxygen tension, fluid flow, and shear stress in these devices. Here, we present a micro-gas exchanger integrated with microfluidics to study RBC adhesion under hypoxic flow conditions mimicking postcapillary venules.

Methods

We simulated a range of physiological conditions and explored RBC adhesion to endothelial or subendothelial components (FN or LN). Blood samples were injected into microchannels at normoxic or hypoxic physiological flow conditions. Quantitative evaluation of RBC adhesion was performed on 35 subjects with homozygous SCD.

Results

Significant heterogeneity in RBC adherence response to hypoxia was seen among

SUBMITTER: Kim M 

PROVIDER: S-EPMC5679205 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature