Splice variants of the extracellular region of RON receptor tyrosine kinase in lung cancer cell lines identified by PCR and sequencing.

ABSTRACT:

Background

Altered expression of receptor tyrosine kinases (RTKs) is a major driver of growth and metastasis of cancers. Recepteur d'origine nantais (RON) receptor is a single-pass transmembrane RTK aberrantly expressed in a number of cancers. Efforts to block deregulated RON signaling in tumors using small molecule kinase inhibitors or antibodies are complicated by the presence of unknown number/types of isoforms of RON, which, despite having similar sequences, are localized differently and mediate varied functions. The objective of this study was to identify splice variants of RON transcripts between exons 1 and 10 that code for the extracellular region.

Methods

Direct cDNA sequencing was performed for the transcript between exons 1-10 of RON by Sanger sequencing in variou

SUBMITTER: Krishnaswamy S 

PROVIDER: S-EPMC5679369 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature