Achieving better-than-3-A resolution by single-particle cryo-EM at 200 keV.

ABSTRACT: Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.

SUBMITTER: Herzik MA 

PROVIDER: S-EPMC5679434 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature