Developing selective media for quantification of multispecies biofilms following antibiotic treatment.

ABSTRACT: The lungs of cystic fibrosis (CF) patients are chronically colonized by a polymicrobial biofilm community, leading to difficult-to-treat infections. To combat these infections, CF patients are commonly treated with a variety of antibiotics. Understanding the dynamics of polymicrobial community composition in response to antibiotic therapy is essential in the search for novel therapies. Culture-dependent quantification of individual bacteria from defined multispecies biofilms is frequently carried out by plating on selective media. However, the influence of the selective agents in these media on quantitative recovery before or after antibiotic treatment is often unknown. In the present study we developed selective media for six bacterial species that are frequently co-isolated from the CF l

SUBMITTER: Vandeplassche E 

PROVIDER: S-EPMC5679531 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature