Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection.

ABSTRACT: The matrix proteins of Staphylococcus aureus biofilm have not been well defined. Previous efforts to identify these proteins were performed using in vitro systems. Here we use a proteomic approach to identify biofilm matrix proteins directly from infected bone implants using a rat model of orthopedic implant-associated S. aureus infection. Despite heavy presence of host proteins, a total of 28 and 105 S. aureus proteins were identified during acute infection and chronic infection, respectively. Our results show that biofilm matrix contains mostly intracellular cytoplasmic proteins and, to a much less extent, extracellular and cell surface-associated proteins. Significantly, leukocidins were identified in the biofilm matrix during chronic infection, suggesting S. aureus is actively attackin

SUBMITTER: Lei MG 

PROVIDER: S-EPMC5679556 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature