Qualitative and quantitative assessment of Illumina's forensic STR and SNP kits on MiSeq FGx™.

ABSTRACT: Massively parallel sequencing (MPS) is a powerful tool transforming DNA analysis in multiple fields ranging from medicine, to environmental science, to evolutionary biology. In forensic applications, MPS offers the ability to significantly increase the discriminatory power of human identification as well as aid in mixture deconvolution. However, before the benefits of any new technology can be employed, a thorough evaluation of its quality, consistency, sensitivity, and specificity must be rigorously evaluated in order to gain a detailed understanding of the technique including sources of error, error rates, and other restrictions/limitations. This extensive study assessed the performance of Illumina's MiSeq FGx MPS system and ForenSeq™ kit in nine experimental runs including 314 reaction

SUBMITTER: Sharma V 

PROVIDER: S-EPMC5679668 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature