Different molecular complexes that mediate transcriptional induction and repression by FoxP3.

ABSTRACT: FoxP3 conditions the transcriptional signature and functional facets of regulatory T cells (T_reg cells). Its mechanism of action, whether as an activator or a repressor, has remained unclear. Here, chromatin analysis showed that FoxP3 bound active enhancer elements, not repressed chromatin, around loci over- or under-expressed in T_reg cells. We evaluated the impact of a panel of FoxP3 mutants on its transcriptional activity and interactions with DNA, transcriptional cofactors and chromatin. Computational integration, confirmed by biochemical interaction and size analyses, showed that FoxP3 existed in distinct multimolecular complexes. It was active and primarily an activator when complexed with the transcriptional factors RELA, IKZF2 and KAT5. In contrast, FoxP3 was i

SUBMITTER: Kwon HK 

PROVIDER: S-EPMC5679728 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature