Senescence-associated ultrastructural features of long-term cultures of induced pluripotent stem cells (iPSCs).

ABSTRACT: Induced pluripotent stem cells (iPSCs) hold great promise for developing personalized regenerative medicine, however characterization of their biological features is still incomplete. Moreover, changes occurring in long-term cultured iPSCs have been reported, suggesting these as a model of cellular aging. For this reason, we addressed the ultrastructural characterization of iPSCs, with a focus on possible time-dependent changes, involving specific cell compartments. To this aim, we comparatively analysed cultures at different timepoints, by an innovative electron microscopic technology (FIB/SEM). We observed progressive loss of cell-to-cell contacts, associated with increased occurrence of exosomes. Mitochondria gradually increased, while acquiring an elongated shape, with well-developed <

SUBMITTER: Colasuonno F 

PROVIDER: S-EPMC5680563 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature