Pancreatic cyst fluid harbors a unique microbiome.

ABSTRACT:

Background

It is clear that specific intestinal bacteria are involved in the development of different premalignant conditions along the gastrointestinal tract. An analysis of the microbial constituents in the context of pancreatic cystic lesions has, however, as yet not been performed. This consideration prompted us to explore whether endoscopically obtained pancreatic cyst fluids (PCF) contain bacterial DNA and to determine the genera of bacteria present in such material.

Methods

Total DNA was isolated from 69 PCF samples. Bacterial 16S rRNA gene-specific PCR was performed followed by Sanger sequencing and de novo deep sequencing for the V3-V4 variable region of 16S rRNA gene.

Results

We observed that 98.2% of the samples were positive in conventional PCR, and that 1

SUBMITTER: Li S 

PROVIDER: S-EPMC5680603 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature