Transplanted human p75-positive stem Leydig cells replace disrupted Leydig cells for testosterone production.

ABSTRACT: Previous studies have demonstrated that rodent stem Leydig cell (SLC) transplantation can partially restore testosterone production in Leydig cell (LC)-disrupted or senescent animal models, which provides a promising approach for the treatment of hypogonadism. Here, we isolated human SLCs prospectively and explored the potential therapeutic benefits of human SLC transplantation for hypogonadism treatment. In adult human testes, p75 neurotrophin receptor positive (p75⁺) cells expressed the known SLC marker nestin, but not the LC lineage marker hydroxysteroid dehydrogenase-3β (HSD3β). The p75⁺ cells which were sorted by flow cytometry from human adult testes could expand in vitro and exhibited clonogenic self-renewal capacity. The p75⁺ cells had multi-lineage

SUBMITTER: Zhang M 

PROVIDER: S-EPMC5680910 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature