Identification of islet-enriched long non-coding RNAs contributing to β-cell failure in type 2 diabetes.

ABSTRACT:

Objective

Non-coding RNAs constitute a major fraction of the β-cell transcriptome. While the involvement of microRNAs is well established, the contribution of long non-coding RNAs (lncRNAs) in the regulation of β-cell functions and in diabetes development remains poorly understood. The aim of this study was to identify novel islet lncRNAs differently expressed in type 2 diabetes models and to investigate their role in β-cell failure and in the development of the disease.

Methods

Novel transcripts dysregulated in the islets of diet-induced obese mice were identified by high throughput RNA-sequencing coupled with de novo annotation. Changes in the level of the lncRNAs were assessed by real-time PCR. The functional role of the selected lncRNAs was determined by modifying their

SUBMITTER: Motterle A 

PROVIDER: S-EPMC5681241 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature