Host-microbiota interaction induces bi-phasic inflammation and glucose intolerance in mice.

ABSTRACT:

Objective

Gut microbiota modulates adiposity and glucose metabolism in humans and mice. Here we investigated how colonization of germ-free (GF) mice affects kinetics of adiposity and glucose metabolism.

Methods

Adiposity and glucose metabolism were evaluated at different time points in ex-GF and antibiotic treated mice after colonization with gut microbiota from a conventionally raised (CONV-R) mouse. Mouse physiology, microbiome configuration, serum cytokine levels, and gene expression for inflammatory markers were performed in different tissues.

Results

Colonization resulted in a bi-phasic glucose impairment: the first phase occurring within 3 days of colonization (early phase) and the second 14-28 days after colonization (delayed phase). The early phase co-occurred

SUBMITTER: Molinaro A 

PROVIDER: S-EPMC5681278 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature