KLK5 induces shedding of DPP4 from circulatory Th17 cells in type 2 diabetes.

ABSTRACT:

Objective

Increasing plasma levels and activity of dipeptidyl peptidase-4 (DPP4 or CD26) are associated with rapid progression of metabolic syndrome to overt type 2 diabetes mellitus (T2DM). While DPP4 inhibitors are increasingly used as anti-hyperglycemic agents, the reason for the increase in plasma DPP4 activity in T2DM patients remains elusive.

Methods

We looked into the source of plasma DPP4 activity in a cohort of 135 treatment naive nonobese (BMI < 30) T2DM patients. A wide array of ex vivo, in vitro, and in silico methods were employed to study enzyme activity, gene expression, subcellular localization, protease identification, surface expression, and protein-protein interactions.

Results

We show that circulating immune cells, particularly CD4+ T cells, served

SUBMITTER: Nargis T 

PROVIDER: S-EPMC5681279 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature