Lactate Promotes Synthetic Phenotype in Vascular Smooth Muscle Cells.

ABSTRACT:

Rationale

The phenotypes of vascular smooth muscle cells (vSMCs) comprise a continuum bounded by predominantly contractile and synthetic cells. Some evidence suggests that contractile vSMCs can assume a more synthetic phenotype in response to ischemic injury, but the mechanisms that activate this phenotypic switch are poorly understood.

Objective

To determine whether lactate, which increases in response to regional ischemia, may promote the synthetic phenotype in vSMCs.

Methods and results

Experiments were performed with vSMCs that had been differentiated from human induced pluripotent stem cells and then cultured in glucose-free, lactate-enriched (L⁺) medium or in standard (L^-) medium. Compared with the L^- medium, the L⁺ me

SUBMITTER: Yang L 

PROVIDER: S-EPMC5681426 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature