First insights into the pleiotropic role of vrf (yedF), a newly characterized gene of Salmonella Typhimurium.

ABSTRACT: Salmonella possesses virulence determinants that allow replication under extreme conditions and invasion of host cells, causing disease. Here, we examined four putative genes predicted to encode membrane proteins (ydiY, ybdJ, STM1441 and ynaJ) and a putative transcriptional factor (yedF). These genes were identified in a previous study of a S. Typhimurium clinical isolate and its multidrug-resistant counterpart. For STM1441 and yedF a reduced ability to interact with HeLa cells was observed in the knock-out mutants, but an increase in this ability was absent when these genes were overexpressed, except for yedF which phenotype was rescued when yedF was restored. In the absence of yedF, decreased expression was seen for: i) virulence-related genes involved in motility, chemotaxis, attachment

SUBMITTER: Balleste-Delpierre C 

PROVIDER: S-EPMC5681696 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature