Production and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidates.

ABSTRACT:

Background

The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus strains. The long alpha helix (LAH) is an attractive vaccine component since it is one of the most conserved influenza hemagglutinin (HA) stalk regions. For an improved immune response, the LAH domain from H3N2 strain has been incorporated into virus-like particles (VLPs) derived from hepatitis B virus core protein (HBc) using recently developed tandem core technology.

Results

Fermentation conditions for recombinant HBc-LAH were established in yeast Pichia pastoris and a rapid and efficient purification method for chimeric VLPs was developed to match the requ

SUBMITTER: Kazaks A 

PROVIDER: S-EPMC5681787 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature