The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1?.
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ABSTRACT: Objective:To investigate the modulated effects of HRF on cyclooxygenase isoform expression and its activity, using the human umbilical vein endothelial cell (HUVEC) model induced by interleukin-1 beta (IL-1?). Methods:Cells were treated with indomethacin (positive control), HRF, and its components at various concentrations prior to treatment with IL-1? at 24?h. Cell viability was determined by MTT assay. Moreover, the anti-inflammatory effects of HRF and its components through mRNA and protein expression were established using real-time quantitative PCR and Western blot, respectively. COX activity was identified via exogenous and endogenous PGE2 productions using the EIA. Result:There was no cytotoxicity in HUVECs treated with HRF. None of the experimental conditions used in the study affected the expression of COX-1, but COX-2 protein expression was inhibited at concentrations under 10?µg/mL. Despite the significantly increased levels of exogenous PGE2, HRF had no effect on COX-2 mRNA expression. However, the production of PGE2 was lower at a concentration of 100?µg/mL HRF than at a concentration below 10?µg/mL. Interestingly, each component of HRF revealed different effects of the Ha-Rak formula. Conclusion:Our preliminary findings suggest that HRF and its components provide diverse modulation of COX-2 and PGE2 at the in vitro level.
SUBMITTER: Palo T
PROVIDER: S-EPMC5682063 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
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