Effect of Isolation Technique and Location on the Phenotype of Human Corneal Stroma-Derived Cells.

ABSTRACT:

Purpose

To determine the effect of the isolation technique and location upon the phenotype of human corneal stroma-derived cells (CSCs).

Methods

CSCs were isolated from the corneal stroma center and periphery using the explant or enzymatic digestion technique. The native tissue was stained for functional markers, while cultured cells were analysed by FACS. PCR was used to determine gene expression in the cultured versus native cells.

Results

The native stroma was positive for α-actinin, ALDH1A1, CD31, CD34, Collagen I, and Vimentin. Cultured cells expressed CD73, CD90, CD105, CD51, Nestin, CD49a, CD49d, ABCG2, and CD47. PCR demonstrated a significant upregulation of ALDH1A1, AQP1, ITGB4, KLF4, CD31, CD34, and CXCR4 in the native tissue, while the expression of

SUBMITTER: Nagymihaly R 

PROVIDER: S-EPMC5682086 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature