Development of amplicon deep sequencing markers and data analysis pipeline for genotyping multi-clonal malaria infections.

ABSTRACT:

Background

Amplicon deep sequencing permits sensitive detection of minority clones and improves discriminatory power for genotyping multi-clone Plasmodium falciparum infections. New amplicon sequencing and data analysis protocols are needed for genotyping in epidemiological studies and drug efficacy trials of P. falciparum.

Methods

Targeted sequencing of molecular marker csp and novel marker cpmp was conducted in duplicate on mixtures of parasite culture strains and 37 field samples. A protocol allowing to multiplex up to 384 samples in a single sequencing run was applied. Software "HaplotypR" was developed for data analysis.

Results

Cpmp was highly diverse (H_e = 0.96) in contrast to csp (H_e = 0.57). Minority clones were robustly detected if thei

SUBMITTER: Lerch A 

PROVIDER: S-EPMC5682641 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature