Comparative Assessment of Anti-HLA Antibodies Using Two Commercially Available Luminex-Based Assays.

ABSTRACT:

Background

Allospecific anti-HLA antibodies (Abs) are associated with rejection of solid organ grafts. The 2 main kits to detect anti-HLA Ab in patient serum are commercialized by Immucor and One Lambda/ThermoFisher. We sought to compare the performance of both platforms.

Methods

Background-adjusted mean fluorescence intensity (MFI) values were used from both platforms to compare sera collected from 125 pretransplant and posttransplant heart and lung transplant recipients.

Results

Most HLA class I (94.5%) and HLA class II (89%) Abs with moderate to high MFI titer (≥4000) were detected by both assays. A modest correlation was observed between MFI values obtained from the 2 assays for both class I (r = 0.3, r² = 0.09, P < 0.0001) and clas

SUBMITTER: Clerkin KJ 

PROVIDER: S-EPMC5682763 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature