Characterization of Pb51 in Plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission.

ABSTRACT:

Background

A vaccine that targets multiple developmental stages of malaria parasites would be an effective tool for malaria control and elimination.

Methods

A conserved gene in Plasmodium, the Plasmodium berghei gene (PBANKA_020570) encoding a 51 kDa protein (pb51 gene), was identified through search of the PlasmoDB database using a combination of expression and protein localization criteria. A partial domain of the Pb51 protein was expressed in a prokaryotic expression system (rPb51) and used for immunization in mice. The protein expression profile and localization were studied by Western blot and indirect immunofluorescence assay (IFA), respectively. The inhibitory effect of the anti-rPb51 antibodies on parasite proliferation was evaluated in erythrocytes in vivo. The tran

SUBMITTER: Wang J 

PROVIDER: S-EPMC5683326 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature