A fragment based method for modeling of protein segments into cryo-EM density maps.

ABSTRACT:

Background

Single-particle analysis of electron cryo-microscopy (cryo-EM) is a key technology for elucidation of macromolecular structures. Recent technical advances in hardware and software developments significantly enhanced the resolution of cryo-EM density maps and broadened the applicability and the circle of users. To facilitate modeling of macromolecules into cryo-EM density maps, fast and easy to use methods for modeling are now demanded.

Results

Here we investigated and benchmarked the suitability of a classical and well established fragment-based approach for modeling of segments into cryo-EM density maps (termed FragFit). FragFit uses a hierarchical strategy to select fragments from a pre-calculated set of billions of fragments derived from structures deposited in

SUBMITTER: Ismer J 

PROVIDER: S-EPMC5683378 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature