Comparison of histone-like HU protein DNA-binding properties and HU/IHF protein sequence alignment.

ABSTRACT:

Background

The structure and function of bacterial nucleoid are controlled by histone-like proteins of HU/IHF family, omnipresent in bacteria and also founding archaea and some eukaryotes.HU protein binds dsDNA without sequence specificity and avidly binds DNA structures with propensity to be inclined such as forks, three/four-way junctions, nicks, overhangs and DNA bulges. Sequence comparison of thousands of known histone-like proteins from diverse bacteria phyla reveals relation between HU/IHF sequence, DNA-binding properties and other protein features.

Methodology and principal findings

Performed alignment and clusterization of the protein sequences show that HU/IHF family proteins can be unambiguously divided into three groups, HU proteins, IHF_A and IHF_B proteins. HU p

SUBMITTER: Kamashev D 

PROVIDER: S-EPMC5683647 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature