Development and analytical performance of a new ARCHITECT automated dipeptidyl peptidase-4 immunoassay.

ABSTRACT:

Background

Dipeptidyl peptidase-4 (DPP-4) may be a suitable biomarker to identify people with severe asthma who have greater activation of the interleukin-13 (IL-13) pathway and may therefore benefit from IL-13-targeted treatments. We report the analytical performance of an Investigational Use Only immunoassay and provide data on the biological range of DPP-4 concentrations.

Methods

We assessed assay performance, utilising analyses of precision, linearity and sensitivity; interference from common endogenous assay interferents, and from asthma and anti-diabetic medications, were also assessed. The assay was used to measure the range of serum DPP-4 concentrations in healthy volunteers and subjects with diabetes and severe, uncontrolled asthma.

Results

The total precisio

SUBMITTER: Hemken PM 

PROVIDER: S-EPMC5683673 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature