Vasoconstriction triggered by hydrogen sulfide: Evidence for Na⁺,K⁺,2Cl^-cotransport and L-type Ca²⁺ channel-mediated pathway.

ABSTRACT:

Objectives

This study examined the dose-dependent actions of hydrogen sulfide donor sodium hydrosulphide (NaHS) on isometric contractions and ion transport in rat aorta smooth muscle cells (SMC).

Methods

Isometric contraction was measured in ring aortas segments from male Wistar rats. Activity of Na⁺/K⁺-pump and Na⁺,K⁺,2Cl^-cotransport was measured in cultured endothelial and smooth muscle cells from the rat aorta as ouabain-sensitive and ouabain-resistant, bumetanide-sensitive components of the ⁸⁶Rb influx, respectively.

Results

NaHS exhibited the bimodal action on contractions triggered by modest depolarization ([K⁺]_o=30 mM). At 10^-4 M, NaHS augmented contractions of in

SUBMITTER: Orlov SN 

PROVIDER: S-EPMC5683885 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature